MOREHEAD, Ky. — A Kentucky clerk is again refusing to issue marriage licenses because of her religious objections to same-sex marriage despite the Supreme Court on Monday night denying an emergency application.

Rowan County Clerk Kim Davis sought to put a lower court ruling on hold pending appeal, and in a one-page order the Supreme Court refused.

On Tuesday, Davis’ office denied licenses to at least two couples “under God’s authority.”

The Supreme Court order marks the first time the issue of same-sex marriage has come back to the justices since they issued an opinion in June clearing the way for same-sex couples to marry nationwide.

Davis has refused to issue any marriage licenses since the decision came down. She is an Apostolic Christian who says she has a sincere religious objection to same-sex marriage. Other clerks in the state have expressed concern, but Davis is the only one turning away eligible couples.

A fellow Kentucky clerk, Casey Davis, has protested alongside Kim Davis and insisted Tuesday that “we’ve not tried to prevent” same-sex marriages, and “we’ve only tried to exercise our First Amendment rights.”

Casey Davis said gay couples could go to another county to get married when a county clerk objects because of religious beliefs.

“There was a lot of people that died for that right and I think we should be able to exercise it,” Casey Davis said.

Casey Davis is now riding his bicycle from Louisville, Ky., to the West Coast to raise awareness for what he described as an assault on religious liberty.

In court papers, lawyers for Kim Davis said her “conscience forbids her from approving a (same-sex marriage) license — because the prescribed form mandates that she authorize the proposed union and issue a license bearing her own name and imprimatur.”

“In her belief,” the lawyers wrote, same-sex marriage “is not, in fact, marriage.”

They said issuing a same-sex license would amount to a “searing act of validation” that would “forever echo in her conscience.”